Composite

Part:BBa_K5159030:Design

Designed by: Tianyue WU   Group: iGEM24_HKUST   (2024-10-02)


pTEFin-GapC-LIP2t-LEU2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1947
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1947
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1947
    Illegal BglII site found at 4179
    Illegal BamHI site found at 1713
    Illegal XhoI site found at 2893
    Illegal XhoI site found at 2922
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1947
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1947
    Illegal NgoMIV site found at 2035
    Illegal AgeI site found at 3507
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2553
    Illegal BsaI site found at 2836
    Illegal BsaI.rc site found at 2
    Illegal BsaI.rc site found at 2751
    Illegal SapI site found at 577
    Illegal SapI site found at 3304


Design Notes

None


Source

Please refer to the sources of each subparts.

References